Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method
Identifieur interne : 000083 ( Main/Exploration ); précédent : 000082; suivant : 000084Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method
Auteurs : Xiaowei Zhang [États-Unis] ; Elaine L. Bearer [États-Unis] ; Adriana T. Perles-Barbacaru [États-Unis] ; Russell E. Jacobs [États-Unis]Source :
- Magnetic Resonance in Medicine [ 0740-3194 ] ; 2010-05.
English descriptors
- KwdEn :
Abstract
Golgi impregnation is unique in its ability to display the dendritic trees and axons of large numbers of individual neurons by histology. Here we apply magnetic resonance microscopy to visualize the neuroanatomy of animal models by combining histologic fixation chemistry with paramagnetic contrast agents. Although there is some differential uptake of the standard small‐molecular‐weight contrast agents by different tissue types, detailed discrimination of tissue architecture in MR images does not approach that of standard histology. Our modified Golgi impregnation method significantly increases anatomic detail in magnetic resonance microscopy images. Fixed mouse brains were treated with a solution containing a paramagnetic contrast agent (gadoteridol) and potassium dichromate. Results demonstrate a specific contrast enhancement likely due to diamagnetic hexavalent chromium undergoing tissue specific reduction to paramagnetic trivalent chromium. This new method dramatically improves neuroanatomical contrast compared to conventional fixation, displaying detail approximating that of histologic specimens at low (4×) magnification. Magn Reson Med 63:1391–1397, 2010. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/mrm.22322
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method</title><author><name sortKey="Zhang, Xiaowei" sort="Zhang, Xiaowei" uniqKey="Zhang X" first="Xiaowei" last="Zhang">Xiaowei Zhang</name></author><author><name sortKey="Bearer, Elaine L" sort="Bearer, Elaine L" uniqKey="Bearer E" first="Elaine L." last="Bearer">Elaine L. Bearer</name></author><author><name sortKey="Perles Arbacaru, Adriana T" sort="Perles Arbacaru, Adriana T" uniqKey="Perles Arbacaru A" first="Adriana T." last="Perles-Barbacaru">Adriana T. Perles-Barbacaru</name></author><author><name sortKey="Jacobs, Russell E" sort="Jacobs, Russell E" uniqKey="Jacobs R" first="Russell E." last="Jacobs">Russell E. Jacobs</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CEB64B9F20DA88A07F5E6E2D59A8A0E545075120</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1002/mrm.22322</idno><idno type="url">https://api.istex.fr/document/CEB64B9F20DA88A07F5E6E2D59A8A0E545075120/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000313</idno><idno type="wicri:Area/Main/Curation">000313</idno><idno type="wicri:Area/Main/Exploration">000083</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">000083</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method</title><author><name sortKey="Zhang, Xiaowei" sort="Zhang, Xiaowei" uniqKey="Zhang X" first="Xiaowei" last="Zhang">Xiaowei Zhang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Imaging Center, Beckman Institute, Caltech, Pasadena, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Bearer, Elaine L" sort="Bearer, Elaine L" uniqKey="Bearer E" first="Elaine L." last="Bearer">Elaine L. Bearer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Imaging Center, Beckman Institute, Caltech, Pasadena, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Pathology and Laboratory Medicine, Alpert Medical School of Brown University, Providence, Rhode Island</wicri:regionArea><placeName><region type="state">Rhode Island</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Pathology and of Neurosurgery, University of New Mexico Health Sciences Center, Albuquerque, New Mexico</wicri:regionArea><placeName><region type="state">Nouveau-Mexique</region></placeName></affiliation></author><author><name sortKey="Perles Arbacaru, Adriana T" sort="Perles Arbacaru, Adriana T" uniqKey="Perles Arbacaru A" first="Adriana T." last="Perles-Barbacaru">Adriana T. Perles-Barbacaru</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Imaging Center, Beckman Institute, Caltech, Pasadena, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Jacobs, Russell E" sort="Jacobs, Russell E" uniqKey="Jacobs R" first="Russell E." last="Jacobs">Russell E. Jacobs</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Imaging Center, Beckman Institute, Caltech, Pasadena, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Magnetic Resonance in Medicine</title><title level="j" type="abbrev">Magn. Reson. Med.</title><idno type="ISSN">0740-3194</idno><idno type="eISSN">1522-2594</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-05">2010-05</date><biblScope unit="volume">63</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1391">1391</biblScope><biblScope unit="page" to="1397">1397</biblScope></imprint><idno type="ISSN">0740-3194</idno></series><idno type="istex">CEB64B9F20DA88A07F5E6E2D59A8A0E545075120</idno><idno type="DOI">10.1002/mrm.22322</idno><idno type="ArticleID">MRM22322</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0740-3194</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Contrast agent</term><term>Golgi stain</term><term>histology</term><term>microMRI</term><term>mouse</term><term>neuroanatomy</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Golgi impregnation is unique in its ability to display the dendritic trees and axons of large numbers of individual neurons by histology. Here we apply magnetic resonance microscopy to visualize the neuroanatomy of animal models by combining histologic fixation chemistry with paramagnetic contrast agents. Although there is some differential uptake of the standard small‐molecular‐weight contrast agents by different tissue types, detailed discrimination of tissue architecture in MR images does not approach that of standard histology. Our modified Golgi impregnation method significantly increases anatomic detail in magnetic resonance microscopy images. Fixed mouse brains were treated with a solution containing a paramagnetic contrast agent (gadoteridol) and potassium dichromate. Results demonstrate a specific contrast enhancement likely due to diamagnetic hexavalent chromium undergoing tissue specific reduction to paramagnetic trivalent chromium. This new method dramatically improves neuroanatomical contrast compared to conventional fixation, displaying detail approximating that of histologic specimens at low (4×) magnification. Magn Reson Med 63:1391–1397, 2010. © 2010 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Nouveau-Mexique</li><li>Rhode Island</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Zhang, Xiaowei" sort="Zhang, Xiaowei" uniqKey="Zhang X" first="Xiaowei" last="Zhang">Xiaowei Zhang</name></region><name sortKey="Bearer, Elaine L" sort="Bearer, Elaine L" uniqKey="Bearer E" first="Elaine L." last="Bearer">Elaine L. Bearer</name><name sortKey="Bearer, Elaine L" sort="Bearer, Elaine L" uniqKey="Bearer E" first="Elaine L." last="Bearer">Elaine L. Bearer</name><name sortKey="Bearer, Elaine L" sort="Bearer, Elaine L" uniqKey="Bearer E" first="Elaine L." last="Bearer">Elaine L. Bearer</name><name sortKey="Jacobs, Russell E" sort="Jacobs, Russell E" uniqKey="Jacobs R" first="Russell E." last="Jacobs">Russell E. Jacobs</name><name sortKey="Perles Arbacaru, Adriana T" sort="Perles Arbacaru, Adriana T" uniqKey="Perles Arbacaru A" first="Adriana T." last="Perles-Barbacaru">Adriana T. Perles-Barbacaru</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Amerique/explor/CaltechV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000083 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000083 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Amerique
|area= CaltechV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:CEB64B9F20DA88A07F5E6E2D59A8A0E545075120
|texte= Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method
}}
| This area was generated with Dilib version V0.6.32. |  |